Biotechnologie et Génétique Ingénierie
Integrated Biotechnologie Fundamentals et Applications
Description
Nouveau developments en Systèmes Biologie, OMICS-es, Synthetic Biologie, Bioinformatics, Automatics, Ordinateur Science, et some crossdisciplinary fields like Cognitive Science, Artificial Intelligence pour dealing avec biological applications, are morphing into a new subject: Integrated Biotechnologie. Integrated Biotechnologie focuses strongly sur le high level “global behavior” de complex systems, which is considered to be more holistic than reductionistic. Integrated Biotechnologie is an interdisciplinary en biotechnology that integrates various disciplines, including genetic engineering, cell culture, bioseparation, mathematics et modeling, bioinformatics, etc, et techniques related to up-, mid-, et down-stream biotechnology, into new one to produce or modify biological products or processes pour improving people’s living standards. Integrated biotechnology is unique en bringing together a wide range de scientists, technologists et engineers from so many disciplines as biology, molecular biology, genetics, microbiology, bioinformatics, mathematics et modeling, bioseparation, computer science, engineering principles et so on, particularly en le application de holistic strategy de le up-, mid-, et down-stream biotechnology. This book provides readers avec guidance sur how to study a complex biological system by taking integrative strategy et how to apply integrated biotechnologies to obtain biological products avec some successful examples. Equipped avec le knowledge about le foundations et tools de integrated biotechnology, it will become practicable pour us to do optimal design et development de bioprocesses from upstream to downstream. This book is a comprehensive textbook de modern biotechnology, le intended readers are graduate students plus academic et professional researchers de life science, medical science, environmental science, food science, agricultural science, bioengineering et biotechnology et so on.
Table des matières
Preface; Acknowledgements; À propos de le Authors; PREAMBLE; What is Life: Le universe et vitalism: Space, time et universe; Vitalism; Life is an integrate body de matter et spirit: Matter, spirit et life; Several issues sur life; Reductionism et Holism: Philosophy et science: Reductionism: Types de reductionism; Reductionism en philosophy et science; Reductionism et life sciences; Limitations de reductionism; Holism: Types de holism; Holism en philosophy et science; Holism et life sciences; Limitations de holism; Holistic et reductionistic approach; What’s Integrated Biotechnologie?: Science, engineering et technology; Moderne life sciences; What’s Integrated Biotechnologie?; Aims, scopes, et roles de Integrated Biotechnologie; Perspectives; References; PART I: FOUNDATIONS IN INTEGRATED BIOTECHNOLOGY: Génétique Ingénierie: DNUn replication et gene transcription: Characteristics de le genetic material; DNUn replication et synthesis; Gene transcription; Protein et its biosynthesis: Protein structure; Protein synthesis/translation; Metabolism: Bioenergetics; Carbohydrate metabolism; Lipid metabolism; Amino acid metabolism; Nucleotide metabolism; Biologique nitrogen fixation; Biosynthesis de typical secondary metabolites; Metabolome; Cell Culture et Fermentation: Metabolic regulatory mechanism: Regulation de enzyme activity; Regulation de enzyme biosynthesisý; Signal transduction systems; Mondial regulatory controls; Regulation de energy charge; Osmotic regulation; Basic approaches en metabolically controlled fermentation; Microbial growth et environmental factors: Bacterial growth curve; Nutritional types de microorganisms; Nutrients et culture media; Effect de environmental factors sur microbial growth; Microbial fermentation: Principes de fermentation technology; Classic fermentation technology; Functional integration techniques; Unit operation integration techniques; Parameter control de fermentation processes: Contrôle de dissolved oxygen; Temperature control; Contrôle de pH; Antifoam control; Bioseparation: Introduction: Le characteristics en downstream bioprocess; Le RIPP scheme; Cell disruption: High-shear mechanical methods; Sonication; Detergent methods; Enzymatic lysis; Osmotic shock; Other cell disruption methods; Precipitation: Centrifugation; Salting out/salting in; Isoelectric precipitation; Precipitation avec organic solvent; Non-ionic hydrophilic polymers as precipitants; Heavy metal salts/polyvalent metallic ions as precipitants; Flocculation by polyelectrolytes; Adsorption: Adsorption mechanism; Adsorption isotherm; Adsorption enthalpy; Adsorption en solution; Adsorbent; Solvent extraction: Eau-organic solvent extraction; Aqueous two-phase extraction; Supercritical fluid extraction; Normal et reverse micelles; Chromatographic separation: General chromatography theory; Ion exchange chromatography; Hydrophobic interaction chromatography; Reverse phase chromatography; Affinity chromatography; Size exclusion chromatography; Thin layer chromatograph; High performance liquid chromatography; Expanded bed adsorption; Membrane separation/filtration: Microfiltration; Ultrafiltration; Nanofiltration; Reverse osmosis; Electrodialysis; Drying: Vacuum freeze drying; Pneumatic drying; Spray drying; Fluidized-bed; Crystallization: Crystallization process; Purification; X-ray analysis de crystals; Integration en downstream bioprocessing; References; PART II: TOOLS IN INTEGRATED BIOTECHNOLOGY: Moderne Biotechniques: Recombinant DNUn technology: Le polymerase chain reaction; Moléculaire cloning; Conventional detection et analytical technology: Gel electrophoresis; Blotting analysis; Le study de gene expressions: Microarrays; RNAi technology; Two-hybrid screening; Phage display; Le structural analysis de biomacromolecules: X-ray crystallography; Nuclear magnetic resonance; DNUn sequencing: Maxam-Gilbert sequencing; Chain-termination method- First generation sequencing; Next generation sequencing; Third generation sequencing; Bioinformatics: Storage et analysis de sequence data: Primary sequence databases et their applications: Sequence alignment et searching: Gene prediction: Genome-wide sequence analysis: Structural bioinformatics; Protein structure databases: Protein structure prediction: Bioinformatics en Transcriptomics; Technologies pour large-scale gene expression: Computational analysis de le SAGE data: Computational analysis de le microarray data: Bioinformatics en Proteomics; Expérimental workflow de proteomics: Proteomics databases: Protein network analysis: Bioinformatics en Metabonomics; Moléculaire interaction systems related metabolic pathways: Metabonomics profiles: Final Words: References: PART III; APPLICATIONS OF INTEGRATED BIOTECHNOLOGY; Microbial Cell Factories-Functional Integration; Strategies applied en microbial cell factories; Empirical approaches-fermentation optimization: Génétique approaches-metabolic engineering: Cybernetics: Prokaryotic cell factories-heterologous protein production en Bacillus subtilis; Properties de Bacillus subtilis: Secretory processes de proteins: Génétique engineering approaches: Prokaryotic cell factories-lactic acid fermentation en L. Lactis; Properties de LAB: Lactose metabolism en LAB: Génétique tools pour LAB: Metabolic engineering de exo-polysaccharides production: Eukaryotic cell factories-Filamentous fungi; Common strategies used en Filamentous fungi: Production de fungal enzymes: Eukaryotic cell factories-Yeast cells; Lab sur Un Chip-Unit Operation Integration; Essentiel components en microfluidics devices; Sample extractor: Mixer: Pump: Valve: Microfabrication techniques; Photolithography: Soft lithography: Stencil Patterning: Applications de LOCs; LOC pour analysis de genomics et proteomics: LOC pour cell-based assays: Advantages et disadvantages de LOCs: High-throughput Screening en Biopharmaceutical Industry; High-throughput screening; Expérimental HTS: Virtual HTS: Protein screening; cDNUn library construction: Conversion de cDNUn library to protein library: HTS assay development: Un captivating biopharmaceutical research paradigm Systèmes biology; Un iterative paradigm linking computer science et biology: Un Disrupting paradigm pour le traditional notion de drug discovery research References: Subject Index